Composite
Part:BBa_K088001:Design
Designed by: Hai Chen Group: iGEM08_CPU-NanJing (2008-10-13)
RBS+lacI+pLac
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1164
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
In the presence of lactose or IPTG, an analog of lactose, LacI is unable to correctly bind and inhibit transcription. This allows BBa_C0012 to be used as a inverter or as a detector of lactose or IPTG.
Source
The pLac regulatory region is a 243 base-pair sequence with two protein binding sites: CAP, which is generally present in E.coli and is assocciated with cell health and availability of glucose., and LacI, the Lac inhibitor BBa_C0012,which binds in an dimerized cooperative manner to inhibit the transcription of the protein that follows.